Determination of Antimicrobial Activity of Mulberry Fruit Extracts against Water-borne Microbial Pathogens Isolated from Different Water Samples
Keywords:
Antimicrobial activity, antibiotics, Morus nigra L, Morus alba L, bacterial strainsAbstract
Mulberry (Morus sp.) is being used worldwide due to its nutritional values and medicinal properties. In the current study, antimicrobial activities of Ripened and Un-Ripened Black and White mulberry fruit extracts are tested against microbial pathogens isolated from different water samples. Aqueous fruit extracts show significant antimicrobial activity with 35mm zone of inhibition by Ripened White Toot (RWT) against V. cholera and 32mm zone of inhibition by Un-Ripened Black Toot (URBT) against M. luteus. All mulberry extracts are considerably active against E. coli and B. thuringiensis. Present findings indicate that mulberry fruit extracts have medicinal potential and will be an excellent choice for the development of alternative antimicrobial drug.
References
[1] W. C. Evans, D. Evans, G. E. Trease and Evans. Pharmacognosy.
WB Saunders. Edinburgh, London, 15th edition, 2002.
[2] P. A. De Smet. The role of plant-derived drugs and herbal medicines
in healthcare drugs, Vol. 54(6), pp. 801-840, 1997.
[3] N. R. Farnsworth and D. D. Soejarto. Global importance of
medicinal plants, Conservation of Medicinal Plants, 1991, pp.25-51.
[4] N. G. Bisset and M. Wichtl. Herbal drugs and
phytopharmaceuticals, medpharm gmbh scientific publishers,
Stuttgart, CRC Press, Boca Raton, 1994, pp. 91-95.
[5] P. K. Mukherjee. Quality control of herbal drugs: an approach of
evaluation of botanicals, New Delhi, Business Horizons Publication,
1st edition, 2002.
[6] World Health Organization. National policy on traditional medicine
and regulation of herbal medicines - Report of a WHO Global
Survey, 2005, pp. 1-168.
[7] A. H. Gilani and A. Rahman. Trends in ethno pharmacology, Journal
of Ethnopharmacol, Vol. 100(1), pp. 43-49, 2005.
[8] M. Athar and M. A. Siddiqi. Reflections on the taxonomy and
distribution of medicinal flowers of Pakistan, SIDA, Contributions
to Botany, Vol. 21(1), pp. 357-368, 2004.
[9] Z. K. Shinwari, M. Rehman, T. Watanabe and Y. Yoshikawa.
Medicinal and aromatic plants of Pakistan (A Pictorial Guide),
Kohat University of Science and Technology, Kohat, Pakistan, pp.
492, 2006.
[10] J. A. Duke and E. S. Ayensu. Medicinal plants of china, Journal of
Botanical Taxonomy and Geobotany, Reference publication, Vol.
20(4), 1985.
[11] World Health Organization. National policy on traditional medicine
and regulation of herbal medicines: Report of a WHO global survey,
2005.
[12] N. Nwachcuku and C. P. Gerba. Emerging waterborne pathogens:
can we kill them all, Current Opinion in Biotechnology, Vol. 15(3),
pp. 175-180, 2005.
[13] N. Nwachuku, C. P. Gerba, A. Oswald and F. D. Mashadi.
Comparative inactivation of adenovirus serotypes by UV light
disinfection, Applied and Environmental Microbiology, Vol. 71(9),
pp. 5633-5636, 2005.
[14] E. J. Dziuban, J. L. Liang, G. F. Craun, V. Hill, P. A. Yu, J. Painter,
M. R. Moore, R. L. Calderon, S. L. Roy and M. J. Beach.
Surveillance for waterborne disease and outbreaks associated with
recreational water, United States, 2003–2004, Morbidity and
Mortality Weekly Report: Surveillance Summaries, Vol. 55(12), pp.
1-30, 2006.
[15] B. Petrini. Mycobacterium marinum: ubiquitous agent of
waterborne granulomatous skin infections, European Journal of
Clinical Microbiology and Infectious Diseases, Vol.25(10), pp. 609-
613, 2006.
[16] H. Westh, C. S. Zinn, V.T. Rosdahl and S. S. Group. An
international multicenter study of antimicrobial consumption and
resistance in staphylococcus aureus isolates from 15 hospitals in 14
countries, Microbial Drug Resistance, Vol. 10(2), pp. 169-176,
2004.
[17] S. Hashemi, A. Nasrollah and M. Rajabi. Irrational antibiotic
prescribing: a local issue or global concern, EXCLI Journal, Vol. 12,
pp. 384, 2013.
[18] D. I. Andersson. Persistence of antibiotic resistant bacteria, Current
Opinion in Microbiology, Vol. 6(5), pp.452-456, 2003.
[19] R. V. Kumar and S. Chauhan. Mulberry: Life enhancer, Journal of
Medicinal Plants Research, Vol. 2(10), pp. 271–278, 2008.
[20] S. Ercisli and E. Orhan. Chemical composition of white (Morus
alba), red (Morus rubra) and black (Morus nigra) mulberry
fruits, Food Chemistry, Vol. 103(4), pp. 1380-1384, 2007.
[21] N. M. A. Hassimotto, M.I. Genovese and F.M. Lajolo.
Identification and characterization of anthocyanins from wild
mulberry (Morus nigra L.) growing in Brazil, Food Science and
Technology International. Vol. 1(1), pp. 17-25, 2007.
[22] D. Gerasopoulos and G. Stavroulakis. Quality characteristics of four
mulberry (Morus sp) cultivars in the area of Chania, Greece, Journal of the Science of Food and Agriculture, Vol. 73(2), pp. 261-264,
1997.
[23] A. A. Miles, S. S. Misra and J.O. Irwin. The estimation of the
bactericidal power of the blood, Epidemiology & Infection, Vol.
38(6), pp.732-49, 1938.
[24] G. J. Hucker. A new modification and application of the Gram stain,
Journal of Bacteriology, Vol. 6(4), pp. 395-397, 1921.
[25] W. Z. Sperber and S. R. Tatini. Interpretation of the tube coagulase
test for identification of Staphylococcus aureus, Applied
Microbiology, Vol. 29(4), pp. 502-505, 1975.
[26] F. Y. Aditi, S. S. Rahman and M. M. Hossain. A study on the
microbiological status of mineral drinking water, The Open
Microbiology Journal, Vol. 11, pp. 1-31, 2017.
[27] H. P. Seeliger. Use of a urease test for the screening and
identification of Cryptococci, Journal of Bacteriology, Vol. 72(2),
pp. 127, 1956.
[28] S.P. Chakraborty, S.K. Mahapatra and S. Roy. Biochemical
characters and antibiotic susceptibility of Staphylococcus aureus
isolates. Asian Pacific Journal of Tropical Biomedicine, Vol. 1(3),
pp. 212-216, 2011.
[29] H. C. Thaker, M. N. Brahmbhatt, J.B. Nayak, and H.C. Thaker.
Isolation and identification of staphylococcus aureus from milk and
milk products and their drug resistance patterns in Anand, Gujarat,
Veterinary World, Vol. 6(1), pp.10-13, 2013.
[30] Clinical and Laboratory Standards Institute. Performance standards
for antimicrobial susceptibility testing; twenty-second informational
supplement, Vol. 31(1), 2012.
[31] N. Khalid, S. A. Fawad and I. Ahmed. Antimicrobial activity
phytochemical profile and trace minerals of black mulberry (Morus
nigra L.) fresh juice, Pakistan, Pakistan Journal of Botany, Vol. 43,
pp. 91-96, 2011.
[32] K. M. Park, J. S. You, H.Y. Lee, N. I. Baek and J. K. Hwang.
Kuwanon G: an antibacterial agent from the root bark of Morus alba
against oral pathogens, Journal of ethno pharmacology, Vol. 84(2,
3), pp.181-185, 2003.
[33] H. Y. Sohn, K. H. Son, C. S. Kwon, G. S. Kwon and S. S. Kang.
Antimicrobial and cytotoxic activity of 18 prenylated flavonoids
isolated from medicinal plants: Morus alba L., morus mongolica
schneider, broussnetia papyrifera (l.) vent sophora flavescens ait and
echinosophora koreensis nakai, Phytomedicine, Vol. 11(7, 8), pp.
666-672, 2004.
[34] O. A. Ayoola, R. A. Baiyewu, J. N. Ekunola, B. A. Olajire, J. A.
Egunjobi, E. O Ayeni and O. O. Ayodele. Phytoconstituent
screening and antimicrobial principles of leaf extracts of two
variants of Morus alba (S30 and S54), African Journal of Pharmacy
and Pharmacology, Vol. 5(19), pp. 2161-2165, 2011.
[35] B. Islam, S. N. Khan, I. Haque, M. Alam, M. Mushfiq and A. U.
Khan. Novel anti-adherence activity of mulberry leaves: inhibition
of Streptococcus mutans biofilm by 1-deoxynojirimycin isolated
from Morus alba, Journal of Antimicrobial Chemotherapy, Vol.
62(4), pp. 751-757, 2008.
[36] M. S. Butt, A. Nazir, M. T. Sultan and K. Schroen. Morus alba L.
nature's functional tonic, Trends in Food Science & Technology,
Vol. 19(10), pp. 505-512, 2008.
[37] D. Yigit, and N. Yigit. Antibacterial activity of black mulberry
(Morus nigra) fruits and leaves, Erzincan University, Journal of
Science and Technology, Vol. 1(1), pp. 39-48, 2008.
WB Saunders. Edinburgh, London, 15th edition, 2002.
[2] P. A. De Smet. The role of plant-derived drugs and herbal medicines
in healthcare drugs, Vol. 54(6), pp. 801-840, 1997.
[3] N. R. Farnsworth and D. D. Soejarto. Global importance of
medicinal plants, Conservation of Medicinal Plants, 1991, pp.25-51.
[4] N. G. Bisset and M. Wichtl. Herbal drugs and
phytopharmaceuticals, medpharm gmbh scientific publishers,
Stuttgart, CRC Press, Boca Raton, 1994, pp. 91-95.
[5] P. K. Mukherjee. Quality control of herbal drugs: an approach of
evaluation of botanicals, New Delhi, Business Horizons Publication,
1st edition, 2002.
[6] World Health Organization. National policy on traditional medicine
and regulation of herbal medicines - Report of a WHO Global
Survey, 2005, pp. 1-168.
[7] A. H. Gilani and A. Rahman. Trends in ethno pharmacology, Journal
of Ethnopharmacol, Vol. 100(1), pp. 43-49, 2005.
[8] M. Athar and M. A. Siddiqi. Reflections on the taxonomy and
distribution of medicinal flowers of Pakistan, SIDA, Contributions
to Botany, Vol. 21(1), pp. 357-368, 2004.
[9] Z. K. Shinwari, M. Rehman, T. Watanabe and Y. Yoshikawa.
Medicinal and aromatic plants of Pakistan (A Pictorial Guide),
Kohat University of Science and Technology, Kohat, Pakistan, pp.
492, 2006.
[10] J. A. Duke and E. S. Ayensu. Medicinal plants of china, Journal of
Botanical Taxonomy and Geobotany, Reference publication, Vol.
20(4), 1985.
[11] World Health Organization. National policy on traditional medicine
and regulation of herbal medicines: Report of a WHO global survey,
2005.
[12] N. Nwachcuku and C. P. Gerba. Emerging waterborne pathogens:
can we kill them all, Current Opinion in Biotechnology, Vol. 15(3),
pp. 175-180, 2005.
[13] N. Nwachuku, C. P. Gerba, A. Oswald and F. D. Mashadi.
Comparative inactivation of adenovirus serotypes by UV light
disinfection, Applied and Environmental Microbiology, Vol. 71(9),
pp. 5633-5636, 2005.
[14] E. J. Dziuban, J. L. Liang, G. F. Craun, V. Hill, P. A. Yu, J. Painter,
M. R. Moore, R. L. Calderon, S. L. Roy and M. J. Beach.
Surveillance for waterborne disease and outbreaks associated with
recreational water, United States, 2003–2004, Morbidity and
Mortality Weekly Report: Surveillance Summaries, Vol. 55(12), pp.
1-30, 2006.
[15] B. Petrini. Mycobacterium marinum: ubiquitous agent of
waterborne granulomatous skin infections, European Journal of
Clinical Microbiology and Infectious Diseases, Vol.25(10), pp. 609-
613, 2006.
[16] H. Westh, C. S. Zinn, V.T. Rosdahl and S. S. Group. An
international multicenter study of antimicrobial consumption and
resistance in staphylococcus aureus isolates from 15 hospitals in 14
countries, Microbial Drug Resistance, Vol. 10(2), pp. 169-176,
2004.
[17] S. Hashemi, A. Nasrollah and M. Rajabi. Irrational antibiotic
prescribing: a local issue or global concern, EXCLI Journal, Vol. 12,
pp. 384, 2013.
[18] D. I. Andersson. Persistence of antibiotic resistant bacteria, Current
Opinion in Microbiology, Vol. 6(5), pp.452-456, 2003.
[19] R. V. Kumar and S. Chauhan. Mulberry: Life enhancer, Journal of
Medicinal Plants Research, Vol. 2(10), pp. 271–278, 2008.
[20] S. Ercisli and E. Orhan. Chemical composition of white (Morus
alba), red (Morus rubra) and black (Morus nigra) mulberry
fruits, Food Chemistry, Vol. 103(4), pp. 1380-1384, 2007.
[21] N. M. A. Hassimotto, M.I. Genovese and F.M. Lajolo.
Identification and characterization of anthocyanins from wild
mulberry (Morus nigra L.) growing in Brazil, Food Science and
Technology International. Vol. 1(1), pp. 17-25, 2007.
[22] D. Gerasopoulos and G. Stavroulakis. Quality characteristics of four
mulberry (Morus sp) cultivars in the area of Chania, Greece, Journal of the Science of Food and Agriculture, Vol. 73(2), pp. 261-264,
1997.
[23] A. A. Miles, S. S. Misra and J.O. Irwin. The estimation of the
bactericidal power of the blood, Epidemiology & Infection, Vol.
38(6), pp.732-49, 1938.
[24] G. J. Hucker. A new modification and application of the Gram stain,
Journal of Bacteriology, Vol. 6(4), pp. 395-397, 1921.
[25] W. Z. Sperber and S. R. Tatini. Interpretation of the tube coagulase
test for identification of Staphylococcus aureus, Applied
Microbiology, Vol. 29(4), pp. 502-505, 1975.
[26] F. Y. Aditi, S. S. Rahman and M. M. Hossain. A study on the
microbiological status of mineral drinking water, The Open
Microbiology Journal, Vol. 11, pp. 1-31, 2017.
[27] H. P. Seeliger. Use of a urease test for the screening and
identification of Cryptococci, Journal of Bacteriology, Vol. 72(2),
pp. 127, 1956.
[28] S.P. Chakraborty, S.K. Mahapatra and S. Roy. Biochemical
characters and antibiotic susceptibility of Staphylococcus aureus
isolates. Asian Pacific Journal of Tropical Biomedicine, Vol. 1(3),
pp. 212-216, 2011.
[29] H. C. Thaker, M. N. Brahmbhatt, J.B. Nayak, and H.C. Thaker.
Isolation and identification of staphylococcus aureus from milk and
milk products and their drug resistance patterns in Anand, Gujarat,
Veterinary World, Vol. 6(1), pp.10-13, 2013.
[30] Clinical and Laboratory Standards Institute. Performance standards
for antimicrobial susceptibility testing; twenty-second informational
supplement, Vol. 31(1), 2012.
[31] N. Khalid, S. A. Fawad and I. Ahmed. Antimicrobial activity
phytochemical profile and trace minerals of black mulberry (Morus
nigra L.) fresh juice, Pakistan, Pakistan Journal of Botany, Vol. 43,
pp. 91-96, 2011.
[32] K. M. Park, J. S. You, H.Y. Lee, N. I. Baek and J. K. Hwang.
Kuwanon G: an antibacterial agent from the root bark of Morus alba
against oral pathogens, Journal of ethno pharmacology, Vol. 84(2,
3), pp.181-185, 2003.
[33] H. Y. Sohn, K. H. Son, C. S. Kwon, G. S. Kwon and S. S. Kang.
Antimicrobial and cytotoxic activity of 18 prenylated flavonoids
isolated from medicinal plants: Morus alba L., morus mongolica
schneider, broussnetia papyrifera (l.) vent sophora flavescens ait and
echinosophora koreensis nakai, Phytomedicine, Vol. 11(7, 8), pp.
666-672, 2004.
[34] O. A. Ayoola, R. A. Baiyewu, J. N. Ekunola, B. A. Olajire, J. A.
Egunjobi, E. O Ayeni and O. O. Ayodele. Phytoconstituent
screening and antimicrobial principles of leaf extracts of two
variants of Morus alba (S30 and S54), African Journal of Pharmacy
and Pharmacology, Vol. 5(19), pp. 2161-2165, 2011.
[35] B. Islam, S. N. Khan, I. Haque, M. Alam, M. Mushfiq and A. U.
Khan. Novel anti-adherence activity of mulberry leaves: inhibition
of Streptococcus mutans biofilm by 1-deoxynojirimycin isolated
from Morus alba, Journal of Antimicrobial Chemotherapy, Vol.
62(4), pp. 751-757, 2008.
[36] M. S. Butt, A. Nazir, M. T. Sultan and K. Schroen. Morus alba L.
nature's functional tonic, Trends in Food Science & Technology,
Vol. 19(10), pp. 505-512, 2008.
[37] D. Yigit, and N. Yigit. Antibacterial activity of black mulberry
(Morus nigra) fruits and leaves, Erzincan University, Journal of
Science and Technology, Vol. 1(1), pp. 39-48, 2008.
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Published
2018-08-28
How to Cite
Shumaila Naz. (2018). Determination of Antimicrobial Activity of Mulberry Fruit Extracts against Water-borne Microbial Pathogens Isolated from Different Water Samples. University of Wah Journal of Science and Technology (UWJST), 2, 19–29. Retrieved from https://uwjst.org.pk/index.php/uwjst/article/view/12
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