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Maize is the 3rd most important agricultural crop in Pakistan with an annual production of 5,701,400 tons. Identification of genetically diverse maize genotype is valuable information which is efficiently used in upcoming maize breeding programs and acquiring specific traits with modification of DNA for different conditions. In current study we reported the genetic diversity among twelve maize genotypes using Randomly Amplified Polymorphic DNA (RAPD). Four oligo decamer primers of were used for the analysis. Young and fresh maize leaves were used for DNA extracted by CTAB extraction procedure. RAPD decamer primers were used for DNA amplification. The PCR results were checked on gel electrophoresis with 1.2% agarose gel having ethidium bromide where DNA bands were separated on the base of size. A total of 301 loci were obtained by using four primers. Bands were score with binary method, 1 for their presence or 0 for the absence. After statistical analysis of all loci, phylogenetic tree was constructed using computer program Dendro UPGMA. The results showed that genotypes FSH-810 and Karamat were the most diverse genotypes having maximum dissimilarity ratio of 66% to each other while Islamabad Gold and Soan-3 were least diverse genotypes with 18% variation. Hence it is recomended that FSH-810 and Karamat genotypes are the ideal candidates for any breeding programs potentially generating maximum variation.
 D. Kumar and A. N. Jhariya. Nutritional, medicinal and economical importance of corn: A mini review, Research Journal of Pharmaceutical Sciences. Vol. 2(7), pp. 7-8, 2013.
 Amanullah, M. Asif, L. K Almas, A. Jan, Z. Shah, H. U. Rahman and S. K. Khalil. Agronomic efficiency and profitability of P-fertilizers applied at different planting densities of maize in Northwest Pakistan, Journal of Plant Nutrition, Vol. 35(3), pp. 331-341, 2012.
 Y. Matsuoka, Y. Vigouroux, M. M. Goodman, J. Sanchez, E. Buckler and J. Doebley. A single domestication for maize shown by multilocus microsatellite genotyping, Proceedings of the National Academy of Sciences, 2002, pp. 6080-6084.
 R. L. Paliwal, G. Granados, H. R. Lafitte, A. D. Violic, J. P. Marathee, Tropical Maize Improvement and Production, 2nd Edition, 2000.
 D. A. Sleper and M. P. John. Breeding field crops, Blackwell publishing, 5th edition, 2006.
 F. Orthoefer, J. Eastman, G. List, P. White and L. A. Johnson. Corn oil: composition, processing and utilization, American Association of Cereal Chemists, Vol. 20, pp. 671-694, 2003.
 F. Oner and H. Aykutlu. The effect of maize-soybean intercropping systems on a set of technological and physiological properties, Applied Ecology and Environmental Research, Vol. 17(2) , pp. 2149-2165, 2019.
 M. M. Shah, S. W. Hassan, K. Maqbool, I. Shahzadi and A. Pervez. Comparisons of DNA marker-based genetic diversity with phenotypic estimates in maize grown in Pakistan, Genetics and Molecular Research, Vol. 9(3), pp. 1936-1945, 2010.
 M. Govindaraj, M. Vetriventhan and M. Srinivasan. Importance of genetic diversity assessment in crop plants and its recent advances: An overview of its analytical perspectives, Genetics Research International, pp. 1-14, 2015.
 E. Paterniani, L. L Nass and M. D. Santos. The value of maize genetic resources for Brazil. Udry, Cv; Duarte, W. Uma. 2000.
 E. S. Buckler, J. M. Thornsberry and S. Kresovich. Molecular diversity, structure and domestication of grasses, Genetics Research. Vol. 77(3), pp. 213-218, 2001.
 A. A. Garcia, L. L. Benchimol, A. M. Barbosa, I. O. Geraldi, C. L. Souza Jr and A. P. Souza. Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines, Genetics and Molecular Biology, Vol. 27(4), pp. 579-588, 2004.
 I. Abuali, A. A. Abdelmula, M. M. Khalafalla, N. B. Hamza, A. H. Abdalla and A. E. Idris. Assessment of yield stability and adaptability of parental inbred lines and F1-hybrids of grain maize using AMMI Analysis, Biotechnology Journal International, Vol. 23, pp. 339-349, 2014.
 M. Govarthanan, S. Arunapriya, A. Guruchandar, T. Selvankumar, N. Gnanasekaran and M. Koildhasa. Genetic variability among Coleus sp studied by RAPD banding pattern analysis, Nature Proceeding’s, Vol. 4, pp. 1-15, 2011.
 B. Hirel and A. Gallais. Nitrogen use efficiency: physiological, molecular and genetic investigations towards crop improvement, Advances in Maize, Vol. 3, pp. 285-310, 2011.
 D. Z. Habash, Z. Kehel and M. Nachit. Genomic approaches for designing durum wheat ready for climate change with a focus on drought, Journal of Experimental Botany, Vol. 60(10), pp. 2805-2815, 2009.
 J. Fu, M. F. Zhang and X. H. Qi. Genetic diversity of traditional Chinese mustard crops Brassica juncea as revealed by phenotypic differences and RAPD markers, Genetic Resources and Crop Evolution, Vol. 53(7), pp. 1513-1519, 2006.
 I. Bauer, S. M. Drinic, M. Filipovic and K. Konstantinov. Genetic characterization of early maturing maize hybrids (Zea mays L.) obtained by protein and RAPD markers, Genetics, Vol. 37(3), pp. 235-243, 2005.
 M. Asif and Y. Z. Mehboob-ur-rahman. Genotyping analysis of six maize (Zea mays L.) hybrid using DNA fingerprinting technology, Pakistan Journal of Botany, Vol. 38(5), pp. 1425-1430, 2006.
 M. J. De Vasconcelos, M. S. Antunes, S. M. Barbosa and C. H. De Carvalho. RAPD analysis of callus regenerated and seed grown plants of maize (Zea mays L.), Brazilian Magazine of Corn and Sorghum, Vol. 7(2), pp. 93-104, 2008.
 N. A. Al-Badeiry, T. K. Merza and A. H. Al-Saadi. Assessment of genetic diversity and relationships among maize (Zea mays L.) varieties in Iraq using random amplified polymorphic DNA (RAPD) markers, Journal of Life Sciences. Vol. 7(12), p. 260, 2013.
 D. Molin, C. J. Coelho, D. S. Maximo, F. S. Ferreira, J. R. Gardingo and R. R. Matiello. Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers, Genetics and Molecular Research. Vol. 12(1), pp. 99-114, 2013.
 B. A. Mrutu, T. Feyissa and J. Ndunguru. Assessment of genetic diversity of maize inbred lines and hybrids in Southern Highlands of Tanzania by using random amplified polymorphic DNA (RAPD) markers, American Journal of Research Communication, Vol. 2, pp. 84-99, 2014.